Cell sheet engineering

Keratinocyte cytoskeletal roles in cell sheet engineering

BACKGROUND There is an increasing need to understand cell-cell interactions for cell and tissue engineering purposes, such as optimizing cell sheet constructs, as well as for examining adhesion defect diseases. For cell-sheet engineering, one major obstacle to sheet function is that cell sheets in suspension are fragile and, over time, will contract. While the role of the cytoskeleton in mainta...

Tissue Engineering Based on Cell Sheet Technology

Recently, cell-based therapies, regenerative medicine, and tissue engineering have been progressing rapidly. With the injection of single cell suspensions, the injected cells are expected to remain around the damaged host tissues for the maintenance and recovery of native functions. Injections of bone marrow cells have been applied in many ways, and achieved some good clinical results. However,...

Cell Sheet Engineering for Periodontal Regeneration

Bone engineering by cell sheet technology to repair mandibular defects

Effectiveness of bone engineering by cell sheet technology to repair canine mandibular injuries was investigated. Cell sheets were produced from canine stem cells cultured by density gradient centrifugation and osteoblast induction. A scaffold surface of polylactic-co-glycolic acid (PLGA) copolymer was wrapped with cell sheets. Mandibular injuries were created in 12 healthy dogs followed by imp...

Pulsatile myocardial tubes fabricated with cell sheet engineering.

BACKGROUND Tissue engineering approaches involving the direct transplantation of cardiac patches have received significant attention as alternative methods for the treatment of damaged hearts. In contrast, we used cardiomyocyte sheets harvested from temperature-responsive culture dishes to create pulsatile myocardial tubes and examined their in vivo function and survival. METHODS AND RESULTS ...